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KMID : 1189419980110010001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
1998 Volume.11 No. 1 p.1 ~ p.22
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Experimental Study of Yongdamsagantang on the Anti-viral Activity and Immune Response to Mice
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Kim Nam-Kwen
Kim Jong-Han
Lim Gyu-Sang
Hwang Choong-Yeon
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Abstract
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During the last few years, nitric oxide(NO) as a potent macrophage-derived effector molecule against a variety of bacteria, parasites, and tumors has received increasing attention. More recent studies suggest that NO also has antiviral effects in both murine and human cells. The objective of the current study was to determine the effect of Yongdamsagantang(YST) on the production of NO. Stimulation of mouse peritoneal macrophages with YST after the treatment of recombinant interferon-γ rlFN-γ resulted in the increased NO synthesis. YST had no effect on NO synthesis by itself. When YST was used in combination with rIFN-γ, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. The optimal effect of YST on NO synthesis was shown 6 hour after treatment with rIFN-γ. This increase in NO synthesis was reflected as increased amount of inducible NO synthase(iNOS) protein. NO production was inhibited by NG-monomethyl-L-arginine. The increased production of NO from rIFN-γ plus YST-stimulated cells was decreased by the treatment with staurosporin. In addition, synergy between rIFN-gγ and YST was mainly dependent on YST-induced tumor necrosis factor-α (TNF-α secretion. These results suggest that the capacity of YST to increase NO production fromrIFN-α -primed mouse peritoneal macrophages is the result of YST-induced TNF-α secretion.
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